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. Author manuscript; available in PMC: 2008 Apr 1.
Published in final edited form as: Radiat Phys Chem Oxf Engl 1993. 2007 Apr;76(4):714–721. doi: 10.1016/j.radphyschem.2006.03.002

Figure 1.

Figure 1

A Absorption spectra of γ-irradiated met Mb measured at 77 K after photobleaching with white light. (1) Met Mb before irradiation; (2) 10 kGy dose; (3) 20 kGy; (4) 40 kGy; (5) 70 kGy. The samples were prepared from the same stock solution and irradiated at 77 K up to the shown dose. B Absorption spectra of met Mb (ferric) and cryoreduced Mb (ferrous) measured at 77 K and shown in millimolar absorption units. These spectra were used as the spectral basis for the cryoreduction yield measurements. Insert: Spectra of irradiated apo Mb at a dose 70 kGy after photobleaching. Protein concentration were: (1) 0.6 mM, (2) 0.15 mM.