Figure 4.

Calculation of the yield of radiolytic reduction from the experimental absorption spectra of γ-irradiated met Mb. (1) Spectrum of met Mb before irradiation; (2) Spectrum after γ-irradiation at 70 kGy and photobleaching with the white light; (3) Background subtracted as described in Materials and Methods; (4) Full line is the spectrum (2) with subtracted background (3); dotted line is the fit of this spectrum with two basis spectra shown in Fig. 1B. (5) Residuals.