Fig. 7. Total NOS activity in UAEC infected with or without adenoviruses.

Subconfluent UAEC were infected with or without sham, A-c-Jun, or S-c-Jun adenoviruses (25 MOI) for 4hr. After 24hr incubation, total cell extracts were prepared. Total NOS activity were measured by the ability of total extracts to convert [³H]-L-arginine to [³H]-citrulline as described in Materials and Methods. Reactions using buffer without cellular proteins were run in parallel and used as assay background. Total NOS activity was expressed as [³H]-citrulline formed (DPM) of each group after subtraction of the background. Results represent data (mean ± SEM) run in duplicate using different cell preparations derived from three pregnant ewes. *P<0.05 vs. controls.