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. Author manuscript; available in PMC: 2009 Feb 1.

Published in final edited form as: Mol Immunol. 2007 Sep 14;45(3):839–848. doi: 10.1016/j.molimm.2006.11.026

A, the prostate cell lines DU145, PC3, PC3/AR+ and LNCaP were treated with Pon A to induce hBD-1 expression for 1–3 days after which MTT assay was performed to determine cell viability. Each bar represents the mean ± S.E.M. of three independent experiments performed in triplicate. B, cells positive for propidium iodide and annexin V were considered apoptotic. Times of induction are shown under each panel. Numbers next to the boxes for each time point represent the percentages of propidium iodide (PI)⁻ annexin V⁺ cells (lower right quadrant), and PI⁺ annexin V⁺ cells (upper right quadrant).

A, the prostate cell lines DU145, PC3, PC3/AR+ and LNCaP were treated with Pon A to induce hBD-1 expression for 1–3 days after which MTT assay was performed to determine cell viability. Each bar represents the mean ± S.E.M. of three independent experiments performed in triplicate. B, cells positive for propidium iodide and annexin V were considered apoptotic. Times of induction are shown under each panel. Numbers next to the boxes for each time point represent the percentages of propidium iodide (PI)⁻ annexin V⁺ cells (lower right quadrant), and PI⁺ annexin V⁺ cells (upper right quadrant).