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. Author manuscript; available in PMC: 2008 Nov 15.
Published in final edited form as: Arch Biochem Biophys. 2007 Sep 15;467(2):283–290. doi: 10.1016/j.abb.2007.09.001

Table 1.

Effect of Different Cations on G6P hydrolysis

Net G6P hydrolysis Percent change vs. 1mM
15 min 30 min 15 min 30 min
Mg2+ 0.25mM 1.14±0.2* 1.90±0.1* 121.8 128.7
1mM 0.94+0.1 1.48+0.2
Mn2+ 0.25mM 1.32+0.2 1.82+0.3 105.6 104.0
1 mM 1.25+0.4 1.75+0.2
Ca2+ 0.25mM 1.14+0.3 1.93+0.2 108.6 109.7
1mM 1.05+0.4 1.76+0.5
Co2+ 0.25mM 1.30+0.2 1.92+0.3 103.2 105.5
1mM 1.26+0.4 1.82+0.2
Ni2+ 0.25mM 1.25+0.3 2.00+0.4 104.2 96.6
1mM 1.20+0.2 2.07+0.5

G6P (5 mM) was added at time = 0 min. Net hydrolysis was calculated vs. the hydrolysis at time = 0 min, and expressed as μmol Pi/mg protein. Hydrolysis at time = 45 min was omitted for simplicity. Data are means +S.E. of 4 different preparations.

*

Statistically significant vs. corresponding values at 1mM.