Table 1.
Net G6P hydrolysis | Percent change vs. 1mM | ||||
---|---|---|---|---|---|
15 min | 30 min | 15 min | 30 min | ||
Mg2+ | 0.25mM | 1.14±0.2* | 1.90±0.1* | 121.8 | 128.7 |
1mM | 0.94+0.1 | 1.48+0.2 | |||
Mn2+ | 0.25mM | 1.32+0.2 | 1.82+0.3 | 105.6 | 104.0 |
1 mM | 1.25+0.4 | 1.75+0.2 | |||
Ca2+ | 0.25mM | 1.14+0.3 | 1.93+0.2 | 108.6 | 109.7 |
1mM | 1.05+0.4 | 1.76+0.5 | |||
Co2+ | 0.25mM | 1.30+0.2 | 1.92+0.3 | 103.2 | 105.5 |
1mM | 1.26+0.4 | 1.82+0.2 | |||
Ni2+ | 0.25mM | 1.25+0.3 | 2.00+0.4 | 104.2 | 96.6 |
1mM | 1.20+0.2 | 2.07+0.5 |
G6P (5 mM) was added at time = 0 min. Net hydrolysis was calculated vs. the hydrolysis at time = 0 min, and expressed as μmol Pi/mg protein. Hydrolysis at time = 45 min was omitted for simplicity. Data are means +S.E. of 4 different preparations.
Statistically significant vs. corresponding values at 1mM.