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. Author manuscript; available in PMC: 2007 Dec 11.
Published in final edited form as: J Neuroimmune Pharmacol. 2007 Mar 8;2(2):184–193. doi: 10.1007/s11481-007-9066-2

Fig. 4.

Fig. 4

Effect of extracellular and intercellular ROS on the activation of N-SMase in human primary oligodendrocytes. Differentiated oligodendrocytes were treated with 300 ìM H2O2(a), the combination of 10 μM hypoxanthine and 1 mU/ml xanthine oxidase (b), 100 μM diamide (c), and 3 mM ATZ (d). At different minute intervals, cellular lysates were prepared and activity of N-SMase was assayed using radiolabeled 14C sphingomyelin as described under “Materials and methods”. Control group served as 100%, and data from other groups were expressed as percentage of control. Results are mean ± SD of three different experiments. ap<0.001 and bp<0.05 vs control.