Figure 5.

Activating mutations in Cat/Raf prevent Reg/Raf suppression. Oocytes were injected with buffer (−) or RNA encoding Reg/Raf (+). Four to eight hours later, oocytes were injected with RNA encoding WT-, YY340,341FF-, or Y340D-Cat/Raf and then scored for GVBD. Oocyte lysates were analyzed by immunoblotting to evaluate the expression level of FLAG-tagged Reg/Raf (Middle) and Cat/Raf (Bottom). Numbers shown represent a compilation of three independent experiments where equivalent amounts of Reg/Raf and Cat/Raf proteins were expressed; the protein analysis is from one typical experiment.