Figure 4.

The percentage change of specific [³H]CFT binding (10 nM) to (filled bars) membranes prepared from or (open bars) intact dissociated HEK 293 cells stably transfected with the indicated DAT mutants resulting from a 2-min application of (Upper) 1 mM MTSET or (Lower) 10 mM MTSES. These concentrations were chosen to normalize for the different reactivities of the reagents with simple sulfhydryls in solution. The means and SEM of 5–11 independent experiments each performed with triplicate determinations are shown.