Table 1.
Binding affinities of RNAs to coat protein mutants
| RNA |
Kd, nM
|
|||||
|---|---|---|---|---|---|---|
| Native | Asn87Ala | Tyr85Phe | Tyr85Ser | Tyr85His | Tyr85His (pH 6.5) | |
| 1 (U) | 4.6*, 2.8† | 3.5, 1.7† | 170* | 300* | 33* | 2.6, 4.0† |
| 2 (A) | 66 | 23 | >103 | 103 | 57 | 26 |
| 3 (G) | 300 | 56 | >103 | >103 | >103 | 200 |
| 4 (C) | 0.038† | 0.028† | 7.5 | 11 | 3.5 | 0.045† |
Kd values were measured in 80 mM KCl/10 mM MgCl2/100 mM K–Hepes, pH 7.5, at 0°C except for Tyr85His (pH 6.5), for which 100 mM K–Mes, pH 6.5, substitutes for Hepes.
*
From LeCuyer et al. (18).
†
Calculated by dividing the experimental off-rate with an on-rate of 108 M−1⋅min−1.