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. 1997 Jan 13;136(1):71–80. doi: 10.1083/jcb.136.1.71

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GFP–PTS1 expression construct. A mutant version (S65T) of the GFP cDNA was cloned into the vector pcDNA3 as described in Materials and Methods. The resulting plasmids either expressed GFP (pGFP) alone or GFP onto which a peroxisomal type I targeting signal (Ser-Lys-Leu-COOH) was fused at its COOH-terminal end (pGFP-PTS1). Depicted is pGFP-PTS1 containing the GFP–PTS1 construct under control of the human cytomegalovirus intermediate early promoter/enhancer region (pCMV). BGH poly A, bovine growth hormone polyadenylation signal; SV40 O/P, SV40 origin and early promoter; NEO r, neomycin-resistance gene; SV40 poly A, SV40 T-antigen polyadenylation signal; Col E1, col E1 origin of replication; AMP r, β-lactamase gene.