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. 1997 Jan 13;136(1):81–93. doi: 10.1083/jcb.136.1.81

Figure 3.

Figure 3

LPC and OA added together compensate the effects of each other on lipid mixing and content mixing mediated by different forms of HA. Fusion of HA-expressing cells (HAb2 [a] and HA300a [b]) and hemifusion of GPI-HA–expressing cells (BHA-PI [c]) with prebound RBCs labeled by either membrane dye R18 or aqueous content marker CF was studied with no added lipids (Control) or in the presence of oleoyl LPC (50 μM), OA (50 μM), or both LPC and OA (same concentrations). Fusion was triggered by a 2-min application of low pH medium (pH 5.2 for HAb2 and HA300a cells, and pH 5.25 for BHA-PI cells). Fusion extents, assayed by fluorescence microscopy as R18 redistribution (hatched bars) or as CF redistribution (open bars), were normalized to those in control experiments: 36.1, 26.8, 13.2, and 17.7% for HAb2, HA300a and BHA-PI (all -R18 assay), and HAb2- CF assay, respectively. Each bar is mean ± SEM, n = 3. Bars which were not statistically different from the corresponding controls are labeled by asterisks. In a, fusion extent for HAb2 (CF assay) in the presence of OA alone was significantly higher than that in the presence of both OA and LPC (P < 0.05).