Figure 6.

The inhibition of pore formation by LPC. Fusion of a HAb2 cell with a single RBC. The arrows indicate the beginning of the application of pH 4.9 solution. Note that these experiments were performed at 33°C rather than at room temperature to accelerate fusion pore formation upon low pH application (Spruce et al., 1989). (a) No LPC added. The increase in capacitance reflects the fusion event. The waiting time between the beginning of low pH application and the onset of the capacitance increase was 16.8 ± 8.9 s (n = 10). (b) Capacitance trace in the presence of 25 μM lauroyl LPC.