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. 1997 Mar 10;136(5):1151–1163. doi: 10.1083/jcb.136.5.1151

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LTBP-1 content of matrix digests prepared from cultures treated with transglutaminase inhibitors. (A) Subconfluent cultures of HT 1080 cells were either untreated or treated with 50 μM MDC, 0.06% DMSO, or 100 μM cystamine during the 3-h pulse with [³⁵S]cysteine and overnight chase. (B) Subconfluent homotypic and heterotypic cultures of BAE and BSM cells were either untreated or MDC-treated (50 μM) during the pulse-chase period as described for HT 1080 cells. Matrix digests were prepared from all cultures and immunoprecipitated using Ab 39 serum. Immunoprecipitates were analyzed by SDS-PAGE followed by fluorography.