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. 1997 Mar 10;136(5):1151–1163. doi: 10.1083/jcb.136.5.1151

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Cross-linking LTBP-1 in BAE CM to UMR-106 ECM by Factor XIIIa. (A) UMR-106 and BAE cultures were metabolically labeled using [³⁵S]cysteine and CM were immunoprecipitated using Ab 39 serum. LTBP-1 immunoprecipitates (IPs) from CM were separated by nonreducing SDS-PAGE and visualized by fluorography. (B) Matrix incorporation reactions were done using metabolically labeled CM from BAE or UMR-106 cells and UMR-106 ECMs. The cross-linking reaction was catalyzed by Factor XIIIa in the absence (−) or presence (+) of 50 μM MDC. Matrix digests were prepared by plasmin digestion and immunoprecipitated using Ab 39 serum. Immunoprecipitates were analyzed by reducing SDS-PAGE and fluorography.