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. 1997 Mar 10;136(5):1099–1108. doi: 10.1083/jcb.136.5.1099

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Sperm binding to agarose beads containing coupled proteins. The sperm-bead binding assay was carried out as described in Materials and Methods. (A) Quantitation of the average number of sperm bound to beads containing various covalently coupled proteins. Binding was measured in the absence or presence of 70 μg/ml of polyclonal anti-gp64 antibody (which recognized both gp64 and gp69). Approximately 0.3–0.4 nmol of protein was coupled per mg of beads. Bars represent SD with n = 30 beads. The effects of the anti-gp64 antibody on gp120/112, gp41, gp37 were not determined (ND). (B) Fluorescence micrographs of sperm binding to beads coated with a 1:1 mixture of gp69 and gp64 (a) or BSA (b). Bar, 20 μm.

Sperm binding to agarose beads containing coupled proteins. The sperm-bead binding assay was carried out as described in Materials and Methods. (A) Quantitation of the average number of sperm bound to beads containing various covalently coupled proteins. Binding was measured in the absence or presence of 70 μg/ml of polyclonal anti-gp64 antibody (which recognized both gp64 and gp69). Approximately 0.3–0.4 nmol of protein was coupled per mg of beads. Bars represent SD with n = 30 beads. The effects of the anti-gp64 antibody on gp120/112, gp41, gp37 were not determined (ND). (B) Fluorescence micrographs of sperm binding to beads coated with a 1:1 mixture of gp69 and gp64 (a) or BSA (b). Bar, 20 μm.