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. 1997 Mar 10;136(5):1099–1108. doi: 10.1083/jcb.136.5.1099

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Effect of crude type I collagenase on the VE proteins and on sperm-egg binding. (A) SDS-PAGE analysis of total VE proteins isolated from a control group of eggs (lane 1) or from eggs treated with crude type–I collagenase (lane 2). The gel was stained with Coomassie blue. Molecular weight standards are marked and shown in lane M. From top: 216, 97, 71, and 44 kD. Arrows in lane 1 indicate gp69 and gp64, respectively. Arrows in lane 2 indicate the two bands at ∼65 and 60 kD which are derived from gp69 and gp64, respectively. (B) Sperm binding levels to control eggs (lane 1) or type I collagenase–treated eggs (lane 2). Bars represent SD with n = 15 eggs.

Effect of crude type I collagenase on the VE proteins and on sperm-egg binding. (A) SDS-PAGE analysis of total VE proteins isolated from a control group of eggs (lane 1) or from eggs treated with crude type–I collagenase (lane 2). The gel was stained with Coomassie blue. Molecular weight standards are marked and shown in lane M. From top: 216, 97, 71, and 44 kD. Arrows in lane 1 indicate gp69 and gp64, respectively. Arrows in lane 2 indicate the two bands at ∼65 and 60 kD which are derived from gp69 and gp64, respectively. (B) Sperm binding levels to control eggs (lane 1) or type I collagenase–treated eggs (lane 2). Bars represent SD with n = 15 eggs.