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. 1997 Mar 10;136(5):1047–1058. doi: 10.1083/jcb.136.5.1047

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Effect of agrin and laminin on megaclusters. Cultures were stained for AChRs and photographed at low magnification. (A) In control cultures each muscle cell typically has one or two megaclusters. (B) After treatment with agrin for 1 d, the muscle cells have many agrin-induced macroclusters but often lack megaclusters. (C) After treatment with 6 nM laminin for 1 d, each muscle cell still has one or two megaclusters. Variable numbers of autofluorescent yolk granules are apparent in the central regions of the cells. The faint outlining of some of the cells in C is due to bleedthrough of the very bright LBS immunofluorescence (not shown) at cell peripheries. Bar, 25 μm.