Figure 9.
Agrin-induced clusters on the dorsal surface. (A1 and A2) βDG and LBS immunofluorescence, revealing several patterned clusters, excellent colocalization, and some decline in the density of microclusters in surrounding regions. (B1 and B2) AChR stain and utrophin immunofluorescence, revealing excellent colocalization. (C1 and C2) AChR stain and PY immunofluorescence, revealing excellent colocalization. The agrin treatment was 3 d in A and B and 2 d in C. Similar results were obtained when the agrin treatment was 1 d. Bar, 5 μm.