Table I.
Inhibition of Agrin–induced Clustering by a Tyrphostin
Treatment | Cells with more than five AChR-containing clusters | Inhibition | ||||
---|---|---|---|---|---|---|
No inhibitor | 100 μM RG50864 | |||||
None | 0.6 ± 0.2% | — | — | |||
(five cultures) | ||||||
6 nM Laminin | 1.9 ± 1.1% | — | — | |||
(four cultures) | ||||||
1/200 Agrin | 96% | 57% | 40.6% | |||
1/200 Agrin | 94% | 21% | 77.7% | |||
1/1000 Agrin | 55% | 8% | 85.5% |
The treatments with laminin and agrin were for 1 d. At least 100 muscle cells were assessed in each culture. All AChR-containing clusters were counted, independent of their size.