Table I.
Yeast Strain List
| Strain | Genotype | Source | ||
|---|---|---|---|---|
| 5962-1-1b | α cdc37-1 his4 ade2 lys2 trp1 tyr1 SUP4 | B. Byers* | ||
| AS126-2b | α mps1-1237 ura3-52 leu2-3, 112 his3Δ200 | This study‡ | ||
| AS138-11a | α cdc37-1 leu2-3, 112 ade2 | This study | ||
| AS146-1b | α cdc37-1 mps1-1 ura3-52 leu2-3, 112 hisΔ200 trp1Δ1; pC166NΔ (CDC37-URA3-2μ) | This study | ||
| AS146-26b | α cdc37-1 ura3-52 leu2-3, 112 lys2 | This study | ||
| AS151-4a | a cdc37-1 mps1-737 ura3-52 leu2-3, 112 his3Δ200 | This study‡ | ||
| AS153-12d | α cdc37-1 mps1-1237 ura3-52 leu2-3, 112 lys2 | This study‡ | ||
| AS156-3b | a cdc37-1 mps1-412 ura3-52 leu2-3, 112 trp1Δ1 lys2 | This study§ | ||
| AS157-10a | a cdc37-1 mps1-3796 ura3-52 leu2-3, 112 | This study‖ | ||
| AS158-2a | a cdc37-1 ura3-52 leu2-3, 112 his3Δ200 lys2 | This study | ||
| AS164-13a | α cdc37-2 mps1-737 ura3-52 leu2-3, 112 lys2 aro1 | This study‡ | ||
| AS165-3a | a cdc37-2 mps1-1237 ura3-52 leu2-3, 112 his3Δ200 aro1 | This study‡ | ||
| AS166-5b | a cdc37-2 mps1-412 ura3-52 leu2-3, 112 trp1 aro1 | This study§ | ||
| AS167-6b | a cdc37-2 mps1-3796 ura3-52 leu2-3, 112 his3Δ200 trp1 | This study‖ | ||
| AS171-3b | a cdc37-2 mps1-1 ura3-52; pC166NΔ (CDC37-URA3-2μ) | |||
| AS177-11b | a cdc37-1 mps2-1 ura3-52 leu2-3, 112 | This study | ||
| AS177-11b/p37 | a cdc37-1 mps2-1 ura3-52 leu2-3, 112; pC166NΔ (CDC37-URA3-2μ) | This study | ||
| AS217-5b | α cdc37-1 mps1-6 ura3-52 leu2-3, 112 | This study ¶ | ||
| AS218-9c | a cdc37-2 mps1-6 ura3-52 trp1Δ1 ; pC165 (CDC37-URA3-2μ) | This study ¶ | ||
| AS230-7a | a URA3-GAL1-NmycMPS1 leu2-3, 112 his3Δ200 trp1Δ1 ade2 | This study | ||
| AS230-7b | α cdc37-1 URA3-GAL1-NmycMPS1 leu2-3, 112 his3Δ 200 trp1Δ 1 ade2 | This study | ||
| AS231-2b | α cdc37-1 URA3-HIS3-ProA-MPS1 leu2-3, 112 | This study | ||
| AS231-2d | α URA3-HIS3-ProA-MPS1 leu2-3, 112 | This study | ||
| D8BX5CA | a/α ura3-52/ura3-52 leu2-3, 112/leu2-3, 112 his3Δ200/his3Δ200 trp1Δ1/trp1 Δ1 | This study | ||
| S288c | α gal2 | R.K. Mortimer** | ||
| SR674-2 | a cdc37-2 hom2 arol arg4 trp1 | S.I. Reed†† | ||
| Wx241-17a | α mps1-1 ura3-52 leu2-3, 112 his3Δ200 | This study | ||
| Wx257-2b | a ura3-52 leu2-3, 112 trp1Δ1 | This study | ||
| Wx257-2d | a ura3-52 leu2-3, 112 his3Δ200 | This study | ||
| Wx257-4c | α ura3-52 leu2-3, 112 his3Δ200 | This study |
*
University of Washington, Seattle, WA.
‡
These mps1 alleles originally isolated by E.A. Siewert, University of Colorado, Boulder, CO.
§
This mps1 allele originally isolated by J.V. Kilmartin, MRC, Cambridge, England.
‖
This mps1 allele originally isolated by T. Huffaker, Cornell University, Ithaca, NY.
¶
This mps1 allele originally isolated by D. Koshland, Carnegie Institute of Washington, Baltimore, MD.
**
University of California, Berkeley, CA.
††
Scripps Research Institute, La Jolla, CA.