Figure 3.

Subcellular distribution of incoming HSV-1 capsids. Conventional immunofluorescence microscopy of Vero cells infected with HSV-1 at an MOI of 50 in the presence of CH. The cells were fixed in 3% PFA, permeabilized with 0.1% TX-100, and labeled with anti-HC, an antibody generated against purified capsids. After 2 h of virus binding at 4°C (0 h), there is no signal. At 1 h PI, numerous cytoplasmic capsids (arrows) distributed within the entire cytoplasm are detectable. At 2 h PI, the capsids begin to accumulate at the nucleus. At 4 h PI, most of the capsids decorate the nuclei. Note that at 1 h, the focus was close to the coverslip, whereas at 2 and 4 h, it was at the nuclear membrane.