Figure 9.

Anti-dynein antibodies label incoming viral HSV-1 capsids. Thawed cryosections of Vero cells infected for 1 (c) or 2 h (a, b, and d–h) in the presence of CH at an MOI of 500 were labeled with rabbit antibodies followed by protein A–5 nm (a–d, and g) or 10 nm gold (e, g, and h). About 5% of the incoming viral capsids (a–f, and h) are labeled by anti-dynein (arrows), an affinity-purified antibody made against a recombinant fragment of dynein heavy. Labeled capsids are localized close to the plasma membrane (g, PM), at the nuclear envelope (c and d), or within the cytoplasm without obvious connection to either one (b). Note that the capsids within the extracellular virions (f) or within the endosomes (g and h, E) are not labeled. Anti-dynein also labels the membranes of mitochondria (a, M), endosomes (h, E), and the Golgi complex (a, G) and diffusely the entire cytoplasm, whereas the mitochondrial matrix (a, b, and d) or the nucleus (d, N) show no labeling for dynein. Bar, 100 nm.