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. 1997 Mar 10;136(5):1037–1045. doi: 10.1083/jcb.136.5.1037

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Current recordings from oocytes expressing the Flag insertion mutants. (a) 200 pg of RNA encoding the wild-type Shaker and insertion mutants were injected into oocytes, which were incubated at 20°C for 24–48 h before recording currents using a two-electrode voltage clamp, as described in Materials and Methods. The currents were elicited by depolarizations from a holding potential of −80 mV to between −30 mV and +30 mV in 10 mV increments. The slowly activating outward currents for pk91, pk333, and pk418 result from calcium activated chloride channels that are endogenously present in oocytes from some frogs. (b) 50 ng of RNA encoding insertion mutant pk300 was injected into oocytes. Incubation and recording conditions were as described in a.

Current recordings from oocytes expressing the Flag insertion mutants. (a) 200 pg of RNA encoding the wild-type Shaker and insertion mutants were injected into oocytes, which were incubated at 20°C for 24–48 h before recording currents using a two-electrode voltage clamp, as described in Materials and Methods. The currents were elicited by depolarizations from a holding potential of −80 mV to between −30 mV and +30 mV in 10 mV increments. The slowly activating outward currents for pk91, pk333, and pk418 result from calcium activated chloride channels that are endogenously present in oocytes from some frogs. (b) 50 ng of RNA encoding insertion mutant pk300 was injected into oocytes. Incubation and recording conditions were as described in a.