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. 1997 Feb 24;136(4):895–906. doi: 10.1083/jcb.136.4.895

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(A) Scheme of the screening procedure applied to identify novel binding proteins of known axon-associated glycoproteins. (B) Identification of binding proteins to immobilized axon-associated proteins. Immunoaffinity-purified proteins were immobilized on ELISA plates (200 ng/well), followed by blocking by BSA and washing. Plates were incubated with detergent extracts of retinas from embryonic day 16, washed several times, and then incubated with supernatants from preselected hybridomas. Binding as indicated by the optical density in ELISA plates is shown for one monolonal antibody, termed 4/1. Bars on top of the columns indicate SEM.