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. 1997 Feb 24;136(4):895–906. doi: 10.1083/jcb.136.4.895

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Binding of CALEB to TN-R or to TN-C analyzed by flow cytometry. CALEB, TN-R, or TN-C were coupled to fluorescent microspheres, mixed, and incubated for 1 h at a 1:1 ratio. Aliquots were analyzed by flow cytometry. Two parametric measurements are documented as contour plots using a logarithmic scale for both axes. The fluorescence intensity corresponds to the size of aggregates. Only beads coated with TN-R (A) or TN-C (B), but not F11 (C), NgCAM (D), or NrCAM (E) formed large mixed aggregates with CALEB. The binding between CALEB and TN-C could be completely blocked by Fab fragments of polyclonal antibodies to TN-C (F).