Figure 3.

Transient association of FBPase with dense structures on the sucrose gradients. Wild-type cells were shifted to glucose for 0, 30, 60, or 90 min at 22°C. Fractions 25–30 were pooled from the S-1000 columns and centrifuged. The pellets were resuspended, loaded onto the top of 20–50% sucrose gradients, and centrifuged at 100,000 g for 20 h. Samples were collected from the top and treated as described in Materials and Methods. The positions of FBPase and CPY at t = 0, 30, 60, or 90 min were followed by immunoblotting with anti-FBPase (a–d) and CPY antibodies (e–h) using ECL reactions.