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. 1997 Feb 24;136(4):803–810. doi: 10.1083/jcb.136.4.803

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Protein staining on the second sucrose gradient. Fractions 10 and 11 from the first sucrose gradient were pooled, pelleted at high speed, and recentrifuged on a second equilibrium sucrose gradient. Fractions were collected from the top and processed as described. Proteins were separated by electrophoresis on 10% SDS-PAGE gels and stained by Coomassie blue R-250.