Figure 6.

Reversible elimination of the sustained phase of Ca_c recovery by blockade of mitochondrial Ca²⁺ extrusion. Cells were coloaded with rhod-2 and Calcium Green esters, examined in the perforated patch clamp configuration, and the data analyzed as in Fig. 4. Responses are representative of five similar experiments. (A) Arrows mark the application of sequential depolarizations of 1.5, 2.0, and 2.5 s duration, applied to a single cell in the presence or absence of perfusion with 10 μM CGP-37157 as indicated. (B) Another cell received 0.5, 1.0, and 1.5 s stimuli before, during, and after application of inhibitor.