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. 1997 Mar 24;136(6):1213–1226. doi: 10.1083/jcb.136.6.1213

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Analysis of ribosome/nascent chain-SRα complex formation. pPl 86 was translated in the presence or absence of 0.25 eq. of RM. Aliquots of the translation were removed and the membrane-bound translation products resolved by sedimentation analysis (lanes 1and 2), or subjected to proteolysis with 100 μg/ml proteinase K for 30 min at 4°C, before sedimentation (lanes 3–5). Fractions were processed as described in the legend to Fig. 3. Paired samples were processed in parallel for immunoblot analysis of SRα.