Figure 5.
Nuclei assembled in an interphase extract containing ΔNLA. (a–c) Conventional fluorescence images of lamin and DNA patterns of a nucleus stained for (a) human LA, (b) Xenopus LB3, and (c) DNA. (d and e) Confocal images of a disrupted nucleus stained for (d) human LA and (e) Xenopus LB3. The endogenous lamin structure has been disrupted and LB3 appears in foci colocalizing with ΔNLA. Bar, 5 μm.