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. 1997 Mar 24;136(6):1201–1212. doi: 10.1083/jcb.136.6.1201

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The addition of ΔNLA to nuclear assembly reactions inhibits DNA replication as shown by the reduced incorporation of biotinylated dUTP. Fluorescence images of the LB3 pattern and biotinylated dUTP incorporation in (a and b) control nuclei and (c–f) nuclei formed in the presence of ΔNLA. (a, c, and e) Immunofluorescence using the antibody against Xenopus LB3; (b, d, and f) Texas red–conjugated streptavidin shows the patterns of incorporation of biotinylated dUTP. Disruption of lamin organization greatly inhibited the incorporation of biotinylated nucleotide when compared with control nuclei. However, all of the lamin-disrupted nuclei do contain a faint punctate nucleoplasmic pattern of labeled nucleotide incorporation that is readily resolved by confocal microscopy. (a–d) Conventional optics (see f). (e and f) Confocal optics. Bar, 5 μm.

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