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. 1997 Mar 24;136(6):1307–1322. doi: 10.1083/jcb.136.6.1307

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Effects of ADF1 on the polymerization of ATP- and ADP-actin. (a) Turbidimetric recording of the spontaneous polymerization of 9.6 μM MgATP-actin in the presence of ADF1. (b) Spontaneous polymerization of 20 μM MgADP-actin in the presence of ADF1. The concentrations of ADF1 (in μM) are indicated on the curves. (c) Critical concentration plots for actin assembly derived from turbidimetric measurements. The extent of turbidity change (A _t= − A _t=0) over the time course of polymerization was plotted for actin alone (○) and actin polymerized in the presence of a saturating (1.5 molar equivalent) amount of ADF (•).