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. 1997 Mar 24;136(6):1307–1322. doi: 10.1083/jcb.136.6.1307

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ADF1 increases the rate of filament growth at the barbed end, not at the pointed end of actin filaments. (a) Barbed end growth. The initial rate of elongation of G-actin (3.3 μM) was measured turbidimetrically at the indicated concentrations of spectrin-actin seeds, and the following concentrations of ADF (μM): ▪, 0; ▵, 0.5; •, 1; ○, 1.5. (Bottom inset) Typical raw data at 0, 1, and 1.5 μM ADF. (Top inset) The increase in slope of the data shown in the main panel was plotted versus the concentration of MgATP-G-actin-ADF complex calculated using Eq. 3, with K = 8 μM. (b) Pointed end growth. The assembly of 6.5 μM MgATP–G-actin was initiated by the addition of 20 nM (1 and 3) or 4 nM (2 and 4) gelsolin-actin seeds in the absence (1 and 2) and in the presence (3 and 4) of 3 μM ADF. The reaction was started by addition of salt. The turbidity was measured before the addition of salt and subtracted from the polymerization time course. The dead time was 5 s.