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. 1997 Mar 24;136(6):1307–1322. doi: 10.1083/jcb.136.6.1307

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ADF1 increases the rate of depolymerization from the pointed ends, not from the barbed ends of actin filaments. (a) Depolymerization from the pointed ends. ADF1 was added at the indicated concentrations to a solution of 3.5 μM F-actin and 7 nM gelsolin. The initial rate of depolymerization was measured turbidimetrically. Typical curves are shown in the inset. 0, no ADF; 1, 0.3 μM; 2, 2.4 μM; 3, 6.5 μM; 4, control curve without ADF in which depolymerization was induced by adding 26 μM Tβ₄. (The time course shown was recorded over 200 min, i.e., with a 10-fold contracted scale.) (b) Depolymerization from the barbed ends. Depolymerization of F-actin (6 μM) was induced by addition of 6 μM DNaseI in the absence (thin line) and in the presence (thick line) of 10 μM ADF.