Table III.
Effect of ADF on the Rate of Propulsion of L. monocytogenes in Platelet Extracts
| Additions | ADF | Rate of Movement | Actin tail length | |||
|---|---|---|---|---|---|---|
| μM | μM/min | μm | ||||
| methyl cellulose 0.13% | 0 | 2.7 ± 0.4 (10) | 28.5 ± 14.8 (10) | |||
| 0.25 | 3.3 ± 0.5 (13) | 8.6 ± 2.9 (15) | ||||
| 0.75 | 5.0 ± 0.5 (8) | 5.3 ± 1.2 (15) | ||||
| No methyl cellulose | 0 | 4 ± 0.7 (10) | 16.3 ± 6.0 (10) | |||
| 0.5 | 5.5 ± 0.9 (9) | 5.9 ± 1 (7) | ||||
| 1 | 10.0 ± 3.5 (5) | 5.0 ± 0.9 (12) | ||||
| 1.5 | cannot be estimated | 3.7 ± 0.6 (10) | ||||
| 3 | cannot be estimated | <1 |
All measurements were made over a period of 30–40 min, starting 30 min after mixing the components of the assay and sealing the samples. Total actin concentration was 6.5 μM. The number of measurements made to derive the average values of the rate of propulsion or the actin tail length is given in parentheses. The same 48-fold dilution of platelet cytoplasm was used in all samples.