Table II.

Checkerboard Analysis of Conditioned Medium from Cultures of Hypertrophic Chondrocytes in Suspension with Ascorbic Acid for ∼3 wk

Lower chamber		Upper chamber
		SFM		10% CM		20% CM		50% CM
SFM		22 ± 2* ‡		18 ± 2		17 ± 1		17 ± 4
10% CM		31 ± 6*		26 ± 3‡		25 ± 4		22 ± 4
20% CM		36 ± 4*		40 ± 6		30 ± 6‡		27 ± 3
50% CM		44 ± 6*		41 ± 4		36 ± 3		32 ± 6‡

F12 culture medium was added with increasing volumes (0/10/20/50%) of CM. The different dilutions were added to the upper or the lower chamber or both. Data were collected and analyzed as described in Materials and Methods.  

^* The dose dependence is shown in the first orthogonal lane.  

^‡ The dose dependence of the chemokinetic component is indicated on the diagonal. SFM, serum-free medium, corresponding to 0% CM.