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. 1997 Jun 16;137(6):1287–1307. doi: 10.1083/jcb.137.6.1287

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Localization of myosin-Iβ in frog saccule by immunoelectron microscopy. (A) Immunoelectron microscopy with rafMIβ and protein A–gold detection showing labeling at stereociliary insertions. Myosin-Iβ is particularly enriched at the rootlet density (arrow). (B) Near-horizontal cross-section through the same region as shown in A, passing from cuticular plate (bottom) to bases of stereocilia (top). (Inset) The plane of section. Label appears where stereocilia join the cuticular plate (arrows) but not above (arrowhead). (C) Gold labeling at pericuticular necklace. SC, supporting cell; HC, hair cell. The hair cell/supporting cell junction is marked by the electron-dense band. (D) Gold labeling at upper end of stereocilia. Bars: (A–C) 1 μm; (D) 500 nm.