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. 1997 Jun 16;137(6):1287–1307. doi: 10.1083/jcb.137.6.1287

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Localization of myosin-VI in frog saccule. (A) Confocal image of Vibratome section of saccular epithelium at low magnification, labeled for myosin-VI. Myosin-VI is found nearly exclusively in hair cells. (B and C) Sections through apical surface of saccular sensory epithelium showing myosin-VI in B and actin in C. Note strong pericuticular necklace labeling (arrow in B) and bright labeling of small bundles (asterisk in C). (D and E) High magnification apical section showing myosin-VI labeling in D and actin labeling in E. Note strong myosin-VI labeling of the pericuticular necklace; actin is excluded from this structure. (F) Vibratome section showing cuticular plate labeling in hair cells. The pericuticular necklace is also visible in cross-section (arrow). (G) Myosin-VI labeling in a dissociated hair cell, fixed before antibody incubation. (H) Myosin-VI labeling in a dissociated hair cell, permeabilized with streptolysin O and incubated with myosin-VI antibody overnight before fixation. Hair cells prepared in this manner had very strong cuticular plate immunoreactivity. (I) High power views of isolated stereocilia labeled for myosin-VI (green) and actin (red). Myosin-VI is enriched at the tapered end of the stereocilia. Bars: (A) 100 μm; (B–H) 10 μm; (I) 5 μm.