Table I.
Antibodies Used in This Study
| Antibody | Source | Raised against | Purified against | Reference | ||||
|---|---|---|---|---|---|---|---|---|
| rafMIβ | rabbit serum | frog myosin-Iβ, | aa 760–1,028/MBP | This study | ||||
| aa 899–1,028/His6 | ||||||||
| 20-3-2 | mouse IgM monoclonal | bovine myosin-Iβ | M.C. Wagner, unpublished data | |||||
| 32A | rabbit serum | chicken myosin-V, | aa 899–1,830/His6 | Espreafico et al., 1992 | ||||
| aa 899–1,830/MBP | ||||||||
| rapMVI | rabbit serum | pig myosin-VI, | aa 1,049–1,254/His6 | Hasson and Mooseker, 1994 | ||||
| aa 1,049–1,254/GST | ||||||||
| mapMVI | mouse serum | pig myosin-VI, | aa 1,049–1,254/His6 | This study | ||||
| aa 1,049–1,254/GST | ||||||||
| rapMVI | rabbit serum | frog myosin-VI, | This study | |||||
| aa 291–305 | ||||||||
| rahMVIIa | rabbit serum | human myosin-VIIa, | aa 877–1,075/His6 | Hasson et al., 1995 | ||||
| aa 877–1,075/GST | ||||||||
| mahMVIIa | mouse serum | human myosin-VIIa, | aa 877–1,075/His6 | This study | ||||
| aa 877–1,075/GST |
aa, start and finish amino acids of recombinant fragments from relevant myosin isozyme; His6, hexahistidine fusion using pQE vectors; MBP, maltose-binding protein fusion using pMAL-p; GST, glutathione-S-transferase fusion using pGEX vectors.