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. 1997 Jun 16;137(6):1265–1278. doi: 10.1083/jcb.137.6.1265

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Isopycnic density gradient analysis of 20KgP fractions of the wild-type and pex16 strains. Strains were grown as described in the legend to Fig. 3 and subjected to subcellular fractionation. The 20KgP from each strain was subjected to centrifugation on a discontinuous sucrose gradient, as described in Materials and Methods. Each gradient was collected in 18 equivolume fractions. (A) Distributions of catalase, fumarase, and protein are shown for the strains E122 (-○-), pex16-1 (-x-), and P16KO-8A (-▪-). The stippled line in the panel at the top shows the density profile (g/cm³) of the gradient. Results are the means of two independent experiments, each analyzed in duplicate. (B) Equal volumes of gradient fractions were analyzed by immunoblotting. Abbreviations are as in Fig. 4.