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. 1997 Jun 16;137(6):1381–1392. doi: 10.1083/jcb.137.6.1381

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Detection of PC-PLC in the supernatant of L. monocytogenes broth cultures. Strain SLCC-5764 and isogenic mutants were used for this experiment. For each assay, an equal volume of concentrated bacterial secreted proteins was loaded per lane. (a) Coomassie blue staining of secreted proteins resolved by SDS-PAGE. (Lane 1) 31-kD protein marker; (lane 2) SLCC5764; (lane 3) DP-L1553 (PI-PLC⁻); (lane 4) DP-L1938 (PIPLC⁻, PC-PLC⁻); (lane 5) DP-L1955 (ActA⁻); (lane 6) DPL1545 (Mpl⁻). (b) Alkaline phosphatase Western immunoblot. (Lane 1) 32-kD prestained molecular mass marker; (lanes 2–6) same as in a. (c) Egg yolk gel overlay assay. (Lane 1) 32- and 42-kD prestained molecular mass markers; (lanes 2–6) same as in a.