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. 1997 Jun 16;137(6):1381–1392. doi: 10.1083/jcb.137.6.1381

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PC-PLC activity in lysates of infected cells. J774 cells were infected with strain 10403S and isogenic mutants. Cells were harvested in 2× SDS sample buffer at 5.5 h after infection. After electrophoresis, the gel was successively soaked in isopropanol and PBS and overlaid with egg yolk agar. Zones of opacity are indicative of phospholipase activity. (Lane 1) Uninfected cells; (lane 2) 10403S; (lane 3) DP-L1935 (PC-PLC⁻); (lane 4) DPL1942 (ActA⁻); (lane 5) DP-L2296 (Mpl⁻). Number of CFU per sample: 1.5 × 10⁸ (lane 2), 2.0 × 10⁸ (lane 3), 2.7 × 10⁸ (lane 4), and 1.9 × 10⁸ (lane 5).