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. 1997 Jun 16;137(6):1381–1392. doi: 10.1083/jcb.137.6.1381

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Determination of proPC-PLC intracellular stability in cells infected with nonspreading bacteria. J774 cells were infected with 10403S isogenic mutants. Cells were pulse labeled for 10 min with [³⁵S]methionine at 3 h and 50 min after infection, and then chased with methionine and chloramphenicol to prevent further incorporation of labeled methionine. Host protease inhibitors were added 1 h before labeling and during labeling at a final concentration of 50 μM. Samples were harvested for PC-PLC immunoprecipitation at specific times during the chase. PC-PLC was detected by fluorography. (Lanes 1–12) DP-L1942 (ActA⁻); (lane 13) DP-L1935 (PC-PLC⁻). Number of CFU per sample was 1.4 × 10⁷.