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. 1997 Jun 16;137(6):1243–1254. doi: 10.1083/jcb.137.6.1243

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Clathrin coats on ANF granules. Sections of atrial tissue fixed without glutaraldehyde and not embedded in gelatin. (A and B) Sections treated as in Fig. 2 B. Coated parts can be seen on granules (arrows), in A, in the cell center (g, Golgi complex) and, in B, near the cell surface (ex, extracellular space). GLUT-4 labeling (10 nm gold) is similar in coated segments and other parts of the granule surface. (C) Section labeled for the TGR-type adaptor protein, AP1. Coats on ANF granules are labeled (10 nm gold) for AP1 (arrows). n, nucleus. Bars, 200 nm.