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. 1997 Jun 16;137(6):1393–1401. doi: 10.1083/jcb.137.6.1393

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Alkaline phosphatase treatment. Anti-occludin pAb (F4 + F5) immunoprecipitates from the total (T), NP-40–soluble (S), and NP-40–insoluble (I) fractions of confluent MDCK I cells were incubated in the presence (+) or absence (−) of alkaline phosphatase (AP) and its specific inhibitor (PI) and then immunoblotted with anti-occludin mAb (MOC37). Alkaline phosphatase significantly decreased the apparent molecular masses of NP-40–soluble and -insoluble occludin bands to the level of the lowest M _r band, and its inhibitor completely suppressed this effect.