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. 1997 Jun 16;137(6):1393–1401. doi: 10.1083/jcb.137.6.1393

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Multiple banding pattern and detergent solubility of occludin in MDCK I cells grown in normal calcium (1.8 mM; NC) or low calcium (5 μM; LC) medium for 24 h. Anti-occludin pAb (F4 + F5) immunoprecipitates from the NP-40–soluble (S) and NP-40–insoluble (I) fractions of confluent MDCK I cells were immunoblotted with anti-occludin mAb (MOC37). Under low calcium conditions, the amount of NP-40–insoluble occludin was fairly small.