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. 1997 Jun 16;137(6):1393–1401. doi: 10.1083/jcb.137.6.1393

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Two distinct anti–chicken occludin mAbs, Oc-2 and Oc-3. Chicken occludin in the isolated junctional fraction was solubilized using 1% SDS, immunoprecipitated with anti–chicken occludin pAb (F44), incubated in the presence (+) or absence (−) of alkaline phosphatase (AP) and its inhibitor (PI), and then immunoblotted with Oc-2 or -3. Oc-3 could not detect dephosphorylated occludin, whereas Oc-2 recognized both phosphorylated and dephosphorylated occludin.