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. 1997 Jun 16;137(6):1337–1354. doi: 10.1083/jcb.137.6.1337

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Upon release from nitrogen starvation, dim1-35 cells incubated at restrictive temperature accumulate 2C DNA but display first cell cycle arrest at or just before entry into mitosis. dim1-35 cells were starved for nitrogen at permissive temperature and then shifted to rich medium at restrictive temperature. Samples were collected for analysis at 30-min intervals. (a) DNA content of dim1-35 cells after shift. (b) Viability (○), 2C DNA content (□), and septation index (•) of dim1-35 cells after shift. Note that viability remains high until the majority of cells have completed S phase (i.e., accumulated 2C DNA) and then begins to decline ∼4 h into the time course, just before septated cells are first observed (at t = 4.5 h). (c) Cut phenotype of septated cells at t = 5 h. Bar, 5 μm.

Upon release from nitrogen starvation, dim1-35 cells incubated at restrictive temperature accumulate 2C DNA but display first cell cycle arrest at or just before entry into mitosis. dim1-35 cells were starved for nitrogen at permissive temperature and then shifted to rich medium at restrictive temperature. Samples were collected for analysis at 30-min intervals. (a) DNA content of dim1-35 cells after shift. (b) Viability (○), 2C DNA content (□), and septation index (•) of dim1-35 cells after shift. Note that viability remains high until the majority of cells have completed S phase (i.e., accumulated 2C DNA) and then begins to decline ∼4 h into the time course, just before septated cells are first observed (at t = 4.5 h). (c) Cut phenotype of septated cells at t = 5 h. Bar, 5 μm.