Table I.
S. Pombe Strains Used in This Study
| Strain designation | Genotype | Source | ||
|---|---|---|---|---|
| KGY28 | h- 972 | P. Nurse* | ||
| KGY56 | h+ nda3-km311 leu1-32 | P. Nurse | ||
| KGY69 | h+ 975 | P. Nurse | ||
| KGY89 | h- nda2-km52 leu1-32 | P. Nurse | ||
| KGY90 | h- dis1-288 leu1-32 | P. Nurse | ||
| KGY107 | h- cdc11-119 | P. Nurse | ||
| KGY155 | h+ cdc25-22 | P. Nurse | ||
| KGY246 | h- ura4-D18 leu1-32 ade6-M210 | P. Nurse | ||
| KGY247 | h+ ura4-D18 leu1-32 ade6-M210 | P. Nurse | ||
| KGY248 | h- ura4-D18 leu1-32 ade6-M216 | P. Nurse | ||
| KGY249 | h+ ura4-D18 leu1-32 ade6-M216 | P. Nurse | ||
| KGY384 | h+ dim1-35 cdc25-22 | This study | ||
| KGY387 | h- dim1-35 dis1-288 | This study | ||
| KGY392 | h+ dim1-35 | This study | ||
| KGY394 | h+ dim1-35 ade6-M216 | This study | ||
| KGY396 | h+ dim1-35 leu1-32 | This study | ||
| KGY402 | h- dim1-35 | This study | ||
| KGY455 | h- cdc2-D217N ura4-D18 leu1-32 ade6-M210 | Berry and Gould, 1996 | ||
| KGY490 | h- dim1-35 ura4-D18 | This study | ||
| KGY517 | h+ rad1::ura4 + ura4-D18 leu1-32 | S. Subramani‡ | ||
| KGY533 | h- cdc2-22 leu1-32 ura4-D18 ade6-M216 | P. Nurse | ||
| KGY557 | h- dim1-35 ade6-M210 | This study | ||
| KGY621 | h- cdc13-A381V his3-237 ura4-D18 leu1-32 ade6-M210 | Berry and Gould, 1996 | ||
| KGY698 | h+ dim1-35 his3-237 ura4-D18 leu1-32 ade6-M210 | This study | ||
| KGY833 | h+/h- dim1-35/dim1::ura4 + his3-237/his3 + ura4-D18/ura4-D18 leu1-32/leu1-32 ade6-M210/ade6-M216 | This study | ||
| KGY838 | h- cdr1-76 leu1-32 ade6-M216 | Young and Fantes, 1987 | ||
| KGY841 | h+/h- dim1 +/dim1::ura4+ his3-237/his3 + ura4-D18/ura4-D18 leu1-32/leu1-32 ade6-M210/ade6-M216 | This study | ||
| KGY842 | h+ dim1::ura4 + carrying pKG709 ura4-D18 leu1-32 ade6-M216 | This study | ||
| KGY843 | h- cdc13-117 ura4-D18 leu1-32 ade6-M210 | P. Nurse | ||
| KGY858 | h+ dim1::ura4 + carrying pKG718 ura4-D18 leu1-32 ade6-M216 | This study | ||
| KGY872 | h- cdc25-22 dim1-35 his3-D1 ura4-D18 leu1-32 ade6-M210 | This study | ||
| KGY874 | h+ cdc13-117 dim1-35 ura4-D18 leu1-32 ade6-M210 | This study | ||
| KGY878 | h- cdc2-22 dim1-35 | This study | ||
| KGY879 | h- dim1-35 cdc13-A381V | This study | ||
| KGY895 | h- dim1-35 cdr1-76 | This study | ||
| KGY896 | h- cdc2-D217N dim1-35 | This study | ||
| KGY939 | h- cut1-RB5 ura4-D18 leu1-32 | I. Hagan§ | ||
| KGY942 | h+ cdc11-119 dim1-35 | This study | ||
| KGY943 | h- cut1-RB5 dim1-35 leu1-32 | This study | ||
| KGY957 | h+ cut3-477 | R. McIntosh‖ | ||
| KGY958 | h- cut1-205 leu1-32 | R. McIntosh | ||
| KGY982 | h+ nuc2-663 leu1-32 ura4-D18 ade6-M210 h+ | P. Nurse | ||
| KGY1019 | h- cut2-364 leu1-32 | R. McIntosh | ||
| KGY1020 | h- cut2-205 dim1-35 | This study | ||
| KGY1021 | h- cut2-364 dim1-35 | This study | ||
| KGY1022 | h- dim1-35 rad1::ura4 + ura4-D18 | This study | ||
| KGY1028 | h+/h- dim1-35/dim1 + his3-D1/his3-D1 ura4-D18/ura4-D18 leu1-32/leu1-32 ade6-M210/ade6-M216 | This study | ||
| KGY1087 | h+/h- dim1-35/dim1::his3 + his3-D1/his3-D1 ura4-D18/ura4-D18 leu1-32/leu1-32 ade6-M210/ade6-M216 | This study | ||
| KGY1088 | h+/h- dim1::his3 + /dim1 + his3-D1/his3-D1 ura4-D18/ura4-D18 leu1-32/leu1-32 ade6-M210/ade6-M216 | This study | ||
| KGY1089 | h- dim1::his3 + carrying pKG897 his3-D1 ura4-D18 leu1-32 ade6-M210 | This study | ||
| KGY1090 | h+ dim1::his3 + carrying pKG898 his3-D1 ura4-D18 leu1-32 ade6-M210 | This study | ||
| KGY1091 | h+ dim1::ura4 + carrying pKG815 ura4-D18 leu1-32 ade6-M216 | This study | ||
| KGY1092 | h+ dim1::ura4 + carrying pKG930 ura4-D18 leu1-32 ade6-M210 | This study | ||
| KGY1115 | h- leu1-32::pKG984 | This study | ||
| KGY1124 | h- dim1-35 leu1-32::pKG984 | This study | ||
| KGY1138 | h- dim1-35::pKG695 ura4-D18 leu1-32 ade6-M210 | This study | ||
| KGY1139 | h+ dim1-35::pKG695 ura4-D18 leu1-32 ade6-M210 | This study | ||
| KGY1155 | h- nda3-km311 dim1-35 leu1-32 | This study | ||
| KGY1156 | h- cut3-447 dim1-35 | This study | ||
| KGY1180 | h+ dim1::his3 + leu1-32::p980 his3-D1 ura4-D18 ade6-M210 | This study | ||
| KGY1216 | h+ leu1-32::0980 ura4-D18 ade6-M210 | This study |
*
Imperial Cancer Research Fund.
‡
University of California, San Diego, CA.
§
University of Manchester, UK.
‖
University of Colorado, Boulder, CO.