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. 1997 Sep 22;138(6):1265–1278. doi: 10.1083/jcb.138.6.1265

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PAK1 and F-actin distribution after PDGF addition. Confocal microscopy was performed on Swiss 3T3 cells after PDGF stimulation for 10 min, as described in Materials and Methods. Red, rhodamine-phalloidin staining of F-actin; green, fluorescein staining of PAK1; yellow, merged images indicating the areas of colocalization. PAK1 and F-actin are colocalized in dorsal ruffles (A and C), membrane ruffles (A and B), and lamellipodia (D). Bar, 15 μm.